Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins

Thạc sĩTrần Thị MinhChi Nguyen Quynh Ho,, Chung Chinh Doan, Son Nghia Hoang, Diem Hong Tran, Long Thanh Le

Khoa Công Nghệ

Thể loại: Bài báo

Sơ lược nội dung

Simulated microgravity (SMG) induced the changes in cell proliferation and cytoskeleton organization, which plays an important factor in various cellular processes. The inhibition in cell cycle progression has been considered to be one of the main causes of proliferation inhibition in cells under SMG, but their mechanisms are still not fully understood. This study aimed to evaluate the effects of SMG on the proliferative ability and cytoskeleton changes of Chang Liver Cells (CCL-13). CCL-13 cells were induced SMG by 3D clinostat for 72 h, while the control group were treated in normal gravity at the same time. The results showed that SMG reduced CCL-13 cell proliferation by an increase in the number of CCL-13 cells in G0/G1 phase. This cell cycle phase arrest of CCL-13 cells was due to a downregulation of cell cycle-related proteins, such as cyclin A1 and A2, cyclin D1, and cyclin-dependent kinase 6 (Cdk6). SMG-exposed CCL-13 cells also exhibited a downregulation of α-tubulin 3 and β-actin which induced the cytoskeleton reorganization. These results suggested that the inhibited proliferation of SMG-exposed CCL-13 cells could be associate with the attenuation of major cell cycle regulators and main cytoskeletal proteins.

Thông tin chung
Thể loại
Bài báo
Năm xuất bản
27 Thg4 2021
Ngôn ngữ gốc
Tiếng Anh
Tạp chí công bố
International Journal of Molecular Sciences
Ấn phẩm số
Vol. 22 No. 9
Loại tạp chí
Danh mục ISI/Scopus
1661-6596 / 1422-0067
Chất lượng

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